logy - Demonstrated scalability of the approach - Validated long-read sequencing capabilities ## Impact This work enabled: - Structural variant detection - Complex genome assembly - Full-length transcript sequencing - Improved genomic analysis ## Patents [Methods and Compositions for Generating Reference Maps for Nanopore-based Polymer Analysis](https://patents.google.com/patent/US20150132745A1/en) App. No. PCT/US/2015/0152495 (2015) 14:T749,

Decoding Long Nanopore Sequencing Reads

Decoding long reads

Summary

With our established ability to sequence with the nanopore MspA, we demonstrated, for the first time, multi-kilobase reads of genomic DNA. This breakthrough showed the potential for long-read sequencing applications.

Authors

Laszlo, A.H., Derrington, I.M., Ross, B.C., Brinkerhoff, H., Adey, A., Nova, I.C., Craig, J.M., Langford, K.W., Samson, J.M., Daza, R., Doering, K., Shendure, J., Gundlach, J.H.

Publication

Published in Nature Biotechnology
DOI: 10.1038/nbt.2950

Technical Innovation

Our research established:

Methods for handling long DNA molecules
Techniques for maintaining read quality
Solutions for data processing challenges

Key Findings

First multi-kilobase DNA reads using nanopore technology
Demonstrated scalability of the approach
Validated long-read sequencing capabilities

Impact

This work enabled:

Structural variant detection
Complex genome assembly
Full-length transcript sequencing
Improved genomi