6456 (2011) 14:T63f,

Nanopore DNA Sequencing with MspA

Overview

We demonstrated that the Nanopore MspA has the requisite sensitivity to sequence DNA and developed methods to enable practical sequencing applications. This work laid the foundation for nanopore-based DNA sequencing technology.

Key Achievements

Proved MspA's sensitivity for DNA sequence detection
Developed practical sequencing methodologies
Established foundation for commercial applications

Technical Innovation

Our research demonstrated:

High sensitivity of MspA to DNA sequences
Practical methods for DNA translocation
Scalable sequencing approach

Impact

This work has enabled:

New DNA sequencing platforms
Rapid, long-read sequencing
Cost-effective genomic analysis

Nanopore DNA sequencing

Derrington, I.M., Collins, M., Manrao, E., Pavlenok, M., Niederweis, M., Gundlach, J.H. (2010). Nanopore DNA sequencing with MspA. PNAS. doi: 10.1073/pnas.1001831107

Patents

Artificial Mycolic Acid Membranes. App. No. PCT/US/2011/025960 Pub. No. 2011/106456 (2011)