Nanopore Tweezers (SPRNT) technology allows us to: - Measure RNA polymerase movement with unprecedented precision - Track enzyme motion in real-time - Study sequence-dependent pausing mechanisms ## Key Findings Our measurements revealed: - Fractional-nucleotide translocation steps - Sequence-dependent pausing behavior - Detailed mechanics of RNA polymerase movement along DNA 14:T6db,

Nanopore tweezers show fractional-nucleotide translocation in sequence-dependent pausing by RNA polymerase

RNA polymerase nanopore measurement

Summary

With Single-Molecule Picometer Resolution Nanopore Tweezers we measure how a RNA polymerase moves with exquisite detail. This research provides unprecedented insights into the mechanics of RNA polymerase movement along DNA.

Authors

Ian C. Nova, Jonathan M. Craig, Abhishek Mazumder, Andrew H. Laszlo, Ian M. Derrington, Matthew T. Noakes, Henry Brinkerhoff, Shuya Yang, Hanif Vahedian-Movahed, Lingting Li, Yu Zhang, Jasmine L. Bowman, Jesse R. Huang, Jonathan W. Mount, Richard H. Ebright, and Jens H. Gundlach

Publication

Published in PNAS

DOI: 10.1073/pnas.2321017121

Technical Innovation

The Single-Molecule Picometer Resolution Nanopore Tweezers (SPRNT) technology allows us to:

Measure RNA polymerase movement with unprecedented precision
Track enzyme motion in real-time
Study sequence-dependent pausing mechanisms

Key Findings

Our measurements revealed:

Fractional-nucleotide translocation steps
Sequence-depend